Article 1
The preparation belonging to the group ‘Enzymes’, as specified in Annex I, is authorised for use without a time limit as an additive in animal nutrition under the conditions laid down in that Annex.
Commission Regulation (EC) No 521/2005 of 1 April 2005 concerning the permanent authorisation of an additive and the provisional authorisation of new uses of certain additives already authorised in feedingstuffs (Text with EEA relevance)
Commission Regulation (EC) No 521/2005 of 1 April 2005 concerning the permanent authorisation of an additive and the provisional authorisation of new uses of certain additives already authorised in feedingstuffs (Text with EEA relevance)
Data as of 2026-07-04 · Compiled from an official source version. Later amendments or repeals may not be reflected; the official text prevails. · Read the official text ↗
Article 2
The preparation belonging to the group ‘Enzymes’, as specified in Annex II, is provisionally authorised for four years as an additive in animal nutrition under the conditions laid down in that Annex.
Article 3
The preparation belonging to the group ‘Micro-organisms’, as specified in Annex III, is authorised provisionally for four years as an additive in animal nutrition under the conditions laid down in that Annex.
Article 4
This Regulation shall enter into force on the third day following that of its publication in the Official Journal of the European Union .
Supplementary provisions
ANNEX ISupplementary provisions
ANNEX I EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation Units of activity/kg of complete feedingstuff Enzymes ‘E 1623 endo-1,3(4)-beta-glucanase EC 3.2.1.6 endo-1,4-beta-xylanase EC 3.2.1.8 Subtilisin EC 3.4.21.62 Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of: Endo-1,3(4)-beta-glucanase: 100 U ( 1 ) /g Endo-1,4-beta-xylanase: 2 500 U ( 2 ) /g Subtilisin: 800 U ( 3 ) /g Chickens for fattening — endo-1,3(4)-beta-glucanase: 25 U — 1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta-glucanase: 25-100 U endo-1,4-beta-xylanase: 625-2 500 U Subtilisin: 200-800 U. 3. For use in compound feed e.g. containing more than 30 % wheat and 10 % barley. Without a time limit endo-1,4-beta-xylanase: 625 U — subtilisin: 200 U —’ ( 1 ) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C. ( 2 ) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C. ( 3 ) 1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C.
ANNEX IISupplementary provisions
ANNEX II No (or EC No) Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation Units of activity/kg of complete feedingstuff Enzymes ‘50 6-phytase EC 3.1.3.26 Preparation of 6-phytase produced by Aspergillus oryzae (DSM 14223) having a minimum activity of: Liquid form: 20 000 FYT ( 1 ) /g Salmonids — 500 FYT — 1. In the directions for use of the additive, indicate the storage temperature and storage life 2. Recommended dose per kg of complete feedingstuff: 500-2 000 FYT. 3. For use in compound feed rich in phytin-bound phosphorus. 5.4.2009’ ( 1 ) 1 FYT is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute from sodium phytate at pH 5,5 and 37 °C.
ANNEX IIISupplementary provisions
ANNEX III No (or EC No) Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU/kg of complete feedingstuff Micro-organisms ‘22 Enterococcus faecium DSM 7134 Preparation of Enterococcus faecium containing a minimum of: Powder: 1 × 10 10 CFU/g additive Granules (micro-encapsulated form): 1 × 10 10 CFU/g additive Chickens for fattening — 0,2 × 10 9 2 × 10 9 In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting. 5.4.2009’
Source: EUR-Lex (Publications Office of the EU), © European Union, reuse permitted under Commission Decision 2011/833/EU.
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