Commission Regulation (EC) No 1206/2005 of 27 July 2005 concerning the permanent authorisation of certain additives in feedingstuffs Text with EEA relevance

• CELEX: Regulation (EC) No 1206/2005
• Articles: 3

Article 1

The preparations belonging to the group ‘Enzymes’, as specified in the Annex, are authorised for use without a time-limit as additives in animal nutrition under the conditions laid down in that Annex.

Article 2

This Regulation shall enter into force on the third day following that of its publication in the Official Journal of the European Union .

Schedules & Appendices

ANNEX

ANNEX

EC No

Additive

Chemical formula, description

Species or category of animal

Maximum age

Minimum content

Maximum content

Other provisions

End of period of authorisation

Units of activity/kg of complete feedingstuff

Enzymes

E 1602

Endo-1,4-beta-glucanase

EC 3.2.1.4

Endo-1,3(4)-beta-glucanase

EC 3.2.1.6

Endo-1,4-beta-xylanase

EC 3.2.1.8

Preparation of endo-1,4-beta-glucanase, endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 74 252) having a minimum activity of:

liquid and granular form:

endo-1,4-beta-glucanase: 8 000 U  ( 1 ) /ml or g

endo-1,3(4)-beta-glucanase: 18 000 U  ( 2 ) /ml or g

endo-1,4-beta-xylanase: 26 000 U  ( 3 ) /ml or g

Turkeys for fattening

—

Endo-1,4-beta-glucanase: 400 U

—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2.

Recommended dose per kg of complete feedingstuff:

endo-1,4-beta-glucanase: 400-800 U

endo-1,3(4)-beta-glucanase: 900-1 800  U

endo-1,4-beta-xylanase: 1 300 -2 600  U

3.

For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 25 % wheat or 20 % barley and 5 % rye

Without a time-limit

Endo-1,3(4)-beta-glucanase: 900 U

—

Endo-1,4-beta-xylanase: 1 300 U

—

E 1604

Endo-1,3(4)-beta-glucanase

EC 3.2.1.6

Endo-1,4-beta-xylanase

EC 3.2.1.8

Preparation of endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanase produced from Penicillium funiculosum (IMI SD101) having a minimum activity of:

powder form:

endo-1,3(4)-beta-glucanase: 2 000  U  ( 4 ) /g

endo-1,4-beta-xylanase: 1 400  U  ( 5 ) /g

liquid form:

endo-1,3(4)-beta-glucanase: 500 U/ml

endo-1,4-beta-xylanase: 350 U/ml

Pigs for fattening

—

Endo-1,3(4)-beta-glucanase: 100 U

—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2.

Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-glucanase: 100 U

endo-1,4-beta-xylanase: 70 U

3.

For use in compound feed rich in non-starch polysaccharides (mainly beta glucans and arabinoxylans), e.g. containing more than 40 % barley or 20 % wheat

Without a time-limit

Endo-1,4-beta-xylanase: 70 U

—

E 1606

Endo-1,4-beta-xylanase

EC 3.2.1.8

Preparation of endo-1,4-beta-xylanase produced by Bacillus subtilis (LMG S-15136) having a minimum activity of:

solid and liquid forms:

100 IU  ( 6 ) /g or ml

Piglets (weaned)

—

10  IU

—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2.

Recommended dose per kg of complete feedingstuff:

endo-1,4-beta-xylanase: 10 IU

3.

For use in compound feed rich in arabinoxylan, e.g. minimum 40 % wheat or barley

4.

For use in weaned piglets up to approximately 35 kg

Without a time-limit

E 1633

Endo-1,3(4)-beta-glucanase

EC 3.2.1.6

Endo-1,4-beta-xylanase

EC 3.2.1.8

Subtilisin

EC 3.4.21.62

Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:

solid form:

endo-1,3(4)-beta-glucanase: 100 U  ( 7 ) /g

endo-1,4-beta-xylanase: 300 U  ( 8 ) /g

subtilisin: 800 U  ( 9 ) /g

Chickens for fattening

—

Endo-1,3(4)-beta-glucanase: 30 U

—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting

2.

Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-glucanase: 30-100 U

endo-1,4-beta-xylanase: 90-300 U

subtilisin: 240-800 U

3.

For use in compound feed, e.g. containing more than 60 % barley

Without a time-limit

Endo-1,4-beta-xylanase: 90 U

—

Subtilisin: 240 U

—

( 1 )   1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from carboxymethylcellulose per minute at pH 5,0 and 40 °C.

( 2 )   1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from barley beta-glucan per minute at pH 5,0 and 40 °C.

( 3 )   1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from oat spelt xylan per minute at pH 5,0 and 40 °C.

( 4 )   1 U is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 5,0 and 50 °C.

( 5 )   1 U is the amount of enzyme which liberates 4,00 micromoles of reducing sugars (maltose equivalents) from birchwood xylan per minute at pH 5,5 and 50 °C.

( 6 )   1 IU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 4,5 and 30 °C.

( 7 )   1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.

( 8 )   1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.

( 9 )   1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C.