Article 1
The preparation belonging to the group ‘Enzymes’, as specified in the Annex, is authorised provisionally for four years as an additive in animal nutrition under the conditions laid down in that Annex.
Commission Regulation (EC) No 1140/2007 of 1 October 2007 concerning the provisional authorisation of a new use of an additive already authorised in feedingstuffs (Text with EEA relevance)
Commission Regulation (EC) No 1140/2007 of 1 October 2007 concerning the provisional authorisation of a new use of an additive already authorised in feedingstuffs (Text with EEA relevance)
Data as of 2026-07-04 · Compiled from an official source version. Later amendments or repeals may not be reflected; the official text prevails. · Read the official text ↗
Article 2
This Regulation shall enter into force on the 20th day following that of its publication in the Official Journal of the European Union .
Supplementary provisions
ANNEXSupplementary provisions
ANNEX EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation Unit of activity/kg of complete feedingstuff Enzymes 53 Endo-1,3(4)-beta-glucanase EC 3.2.1.6 Endo-1,4-beta-glucanase EC 3.2.1.4 Alpha-amylase EC 3.2.1.1 Bacillolysin EC 3.4.24.28 Endo-1,4-beta-xylanase EC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) and endo-1,4-beta-xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of: Endo-1,3(4)-beta-glucanase: 2 350 U/g ( 1 ) Endo-1,4-beta-glucanase: 4 000 U/g ( 2 ) Alpha-amylase: 400 U/g ( 3 ) Bacillolysin: 450 U/g ( 4 ) Endo-1,4-beta-xylanase: 20 000 U/g ( 5 ) Laying hens — Endo-1,3(4)-beta-glucanase: 587 Endo-1,4-beta-glucanase: 1 000 U Alpha-amylase: 100 U Bacillolysin: 112 U Endo-1,4-beta-xylanase: 5 000 U — 1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kilogram of complete feedingstuff: — endo-1,3(4)-beta-glucanase: 587-2 350 U — endo-1,4-beta-glucanase: 1 000 -4 000 U — alpha-amylase: 100-400 U — bacillolysin: 112-450 U — endo-1,4-beta-xylanase: 5 000 -20 000 U 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and especially arabinoxylans), e.g. containing more than 30 % of wheat. 22 October 2011 ( 1 ) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30 °C. ( 2 ) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 4,8 and 50 °C. ( 3 ) 1 U is the amount of enzyme which liberates 1 micromole of glucose from a cross-linked starch polymer per minute at pH 7,5 and 37 °C. ( 4 ) 1 U is the amount of enzyme which solubilises one microgram of azo-casein substrate per minute at pH 7,5 and 37 °C. ( 5 ) 1 U is the amount of enzyme which liberates 0,0067 micromoles of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 5,3 and 50° C.
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