Commission Regulation (EU) No 8/2010 of 23 December 2009 concerning the authorisation of the serine protease produced by Bacillus licheniformis (DSM 19670) as a feed additive for chickens for fattening (holder of authorisation DSM Nutritional Products Ltd, represented by DSM Nutritional Products Sp.Z.o.o) Text with EEA relevance
Data as of 2026-07-04 · Compiled from an official source version. Later amendments or repeals may not be reflected; the official text prevails. · Read the official text ↗
The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘digestibility enhancers’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union .
ANNEX Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation Units of activity/kg of complete feedingstuff with a moisture content of 12 % Category of zootechnical additives. Functional group: digestibility enhancers 4a13 DSM Nutritional Products Ltd, represented by DSM Nutritional Products Sp.Z.o.o. Serine protease EC 3.4.21.- Additive composition: Preparation of serine protease (EC 3.4.21.-) produced by Bacillus licheniformis (DSM 19670) having a minimum activity of 75 000 PROT ( 1 ) /g Characterisation of the active substance: Serine protease (EC 3.4.21.-) produced by Bacillus licheniformis (DSM 19670) Analytical method ( 2 ) : Colorimetric method measuring yellow complex para-nitroaniline (pNA) released by the enzyme from ‘Suc-Ala-Ala-Pro-Phe-pNA’ at pH 9,0 and at 37 °C Chickens for fattening — 15 000 PROT — 1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. For safety reasons: breathing protection, glasses and gloves shall be used during handling. 13.1.2020 ( 1 ) 1 PROT is the amount of enzyme that releases 1 μmol of p-nitroaniline from 1mM substrate (Suc-Ala-Ala-Pro-Phe-pNA) per minute at pH 9,0 and temperature 37 °C. ( 2 ) Details of the analytical methods are available at the following address of the Community Reference Laboratory: www.irmm.jrc.be/crl-feed-additives
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