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Commission Implementing Regulation (EU) No 302/2014 of 25 March 2014 concerning the authorisation of a preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 126896) as a feed additive for chickens for fattening and weaned piglets (holder of the authorisation ROAL Oy) Text with EEA relevance

Commission Implementing Regulation (EU) No 302/2014 of 25 March 2014 concerning the authorisation of a preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 126896) as a feed additive for chickens for fattening and weaned piglets (holder of the authorisation ROAL Oy) Text with EEA relevance

Implementing Regulation (EU) No 302/2014 · Regulation · 3 articles

Data as of 2026-07-04 · Compiled from an official source version. Later amendments or repeals may not be reflected; the official text prevails. · Read the official text ↗

Authorisation

Article 1

The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘digestibility enhancers’, is authorised as an additive in animal nutrition subject to the conditions laid down in that Annex.

Article 2

This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.

Supplementary provisions

ANNEXSupplementary provisions

ANNEX Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation Units of activity/kg of complete feedingstuff with a moisture content of 12 % Category of zootechnical additives. Functional group: digestibility enhancers 4a20 ROAL Oy Endo-1,3(4)-beta-glucanase EC 3.2.1.6   Additive composition Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 126896) having a minimum activity of: — solid form: endo-1,3(4)-beta-glucanase 200 000 BU  ( 1 ) /g, — liquid form: endo-1,3(4)-beta-glucanase 400 000 BU/ml.   Characterisation of the active substance endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 126896)   Analytical method  ( 2 ) For the quantification of endo-1,3(4)-beta-glucanase activity: spectrophotometric (DNS) method, based on the quantification of released sugars produced by the action of endo-1,3(4)-beta-glucanase on barley beta-glucan at pH 4,8 and 50 °C. Chickens for fattening — 20 000 BU — 1. In the directions for use of the additive and premixture, indicate the storage conditions and stability to pelleting. 2. For use in (weaned) piglets up to approximately 35 kg. 3. For safety: breathing protection, glasses and gloves shall be used during handling. 15 April 2024 Piglets (weaned) 10 000 BU ( 1 )   1 BU is the amount of enzyme which liberates 1 nanomole of reducing sugars (expressed as glucose equivalents) from barley beta-glucan substrate per second at 50 °C and pH 4,8. ( 2 )   Details of the analytical methods are available at the following address of the Reference Laboratory: http://irmm.jrc.ec.europa.eu/EURLs/EURL_feed_additives/Pages/index.aspx

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Source: EUR-Lex (Publications Office of the EU), © European Union, reuse permitted under Commission Decision 2011/833/EU.

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