Commission Implementing Regulation (EU) 2015/1426 of 25 August 2015 concerning the authorisation of the preparation of benzoic acid, thymol, eugenol and piperine as a feed additive for chickens for fattening, chickens reared for laying, minor poultry species for fattening and reared for laying (holder of the authorisation DSM Nutritional Product) (Text with EEA relevance)

• CELEX: Implementing Regulation (EU) 2015/1426
• Articles: 3

Article 1

The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘other zootechnical additives’, is authorised as an additive in animal nutrition, subject to the conditions laid down in that Annex.

Article 2

This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union .

Schedules & Appendices

ANNEX

ANNEX

Identification number of the additive

Name of the holder of authorisation

Additive

Composition, chemical formula, description, analytical method

Species or category of animal

Maximum age

Minimum content

Maximum content

Other provisions

End of period of authorisation

mg/kg of complete feedingstuff with a moisture content of 12 %

Category of zootechnical additives. Functional group: other zootechnical additives (improvement of zootechnical parameters).

4d10

DSM Nutritional Products AG represented in the EU by DSM Nutritional Products Sp. z o.o. Poland

Preparation of benzoic acid, thymol, eugenol, piperine

Additive composition

Preparation of benzoic acid, thymol, eugenol, piperine having a content of:

—

benzoic acid 80 %-83 %

—

thymol 1 %-1,9 %

—

eugenol 0,5 %-1 %

—

piperine 0,05 %-0,1 %

—

benzyl salicylate, iso-amyl salicylate and trans-anethole ≤ 0,6 %

Characterisation of the active substances

benzoic acid (≥ 95,5 % purity): C 7 H 6 O 2 CAS number: 65-85-0

thymol  ( 1 ) : C 10 H 14 O CAS number 89-83-8

eugenol  ( 1 ) : C 10 H 12 O 2 CAS number 97-53-0

piperine  ( 1 ) : C 17 H 19 O 3 N CAS number 94-62-2

Isoamyl salicylate  ( 1 ) CAS number 87-20-7

Benzyl salicylate  ( 1 ) CAS number 118-58-1

Trans-anethole  ( 1 ) CAS number 4180-23-8

Analytical method

( 2 )

For the determination of benzoic acid in the feed additive and premixtures:

—

Reverse Phase High-Performance Liquid Chromatography coupled to an Ultraviolet detector (RP-HPLC-UV)

For the determination of benzoic acid in compound feed:

—

Gas Chromatography Isotope Dilution Mass Spectrometry (GC-IDMS)

For the determination of thymol, eugenol and piperine in the feed additive:

—

Gas Chromatography coupled to a Flame Ionisation Detector (GC-FID)

Chickens for fattening

Chickens reared for laying

Minor poultry species for fattening and reared for laying

—

—

300

1.

In the directions for use of the additive and premixture, indicate the storage conditions and stability to pelleting.

2.

The additive shall not be used with other sources of benzoic acid or benzoates.

3.

For safety: breathing protection, glasses and gloves shall be used during handling.

4.

Minimum recommended dose: 300 mg/kg of complete feedingstuff

15.9.2025

( 1 )   JECFA, Online Edition: ‘Specifications for Flavourings’: http://www.fao.org/ag/agn/jecfa-flav/index.html#T

( 2 )   Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports