Commission Implementing Regulation (EU) 2017/1008 of 15 June 2017 concerning the authorisation of the preparation of of Lactococcus lactis PCM B/00039, Carnobacterium divergens PCM KKP 2012p, Lactobacillus casei PCM B/00080, Lactobacillus plantarum PCM B/00081 and Saccharomyces cerevisiae PCM KKP 2059p as a feed additive for chickens for fattening (holder of authorisation JHJ Ltd) (Text with EEA relevance. )

• CELEX: Implementing Regulation (EU) 2017/1008
• Articles: 3

Article 1

The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘gut flora stabilisers’, is authorised as an additive in animal nutrition.

Article 2

This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union .

Schedules & Appendices

ANNEX

ANNEX

Identification number of the additive

Name of the holder of authorisation

Additive

Composition, chemical formula, description, analytical method

Species or category of animal

Maximum age

Minimum content

Maximum content

Other provisions

End of period of authorisation

CFU//kg of complete feedingstuff with a moisture content of 12 %

Category of zootechnical additives. Functional group: gut flora stabilisers

4b1892

JHJ Ltd

Lactococcus lactis PCM B/00039, Carnobacterium divergens PCM KKP 2012p, Lactobacillus casei PCM B/00080, Lactobacillus plantarum PCM B/00081 and Saccharomyces cerevisiae PCM KKP 2059p

Additive composition

Preparation of Lactococcus lactis PCM B/00039, Carnobacterium divergens PCM KKP 2012p, Lactobacillus casei PCM B/00080, Lactobacillus plantarum PCM B/00081 and Saccharomyces cerevisiae PCM KKP 2059p containing a minimum of 1,2 × 10 9 CFU/g of total Lactic Acid Bacteria (LAB) and Saccharomyces cerevisiae PCM KKP 2059p 1 × 10 7 CFU/g

having a minimum of:

Lactococcus lactis PCM B/00039 ≥ 5 × 10 8 CFU/g

Carnobacterium divergens PCM KKP 2012p ≥ 3 × 10 8 CFU/g

Lactobacillus casei PCM B/00080 ≥ 1 × 10 8 CFU/g

Lactobacillus plantarum PCM B/00081 ≥ 3 × 10 8 CFU/g

Saccharomyces cerevisiae PCM KKP 2059p ≥ 1 × 10 7 CFU/g

Characterisation of the active substance

Viable cells of Lactococcus lactis PCM B/00039, Carnobacterium divergens PCM KKP 2012p, Lactobacillus casei PCM B/00080, Lactobacillus plantarum PCM B/00081 and Saccharomyces cerevisiae PCM KKP 2059p

Analytical method

( 1 )

For the enumeration of Lactococcus lactis PCM B/00039 and Carnobacterium divergens PCM KKP 2012p in the feed additive and feedingstuffs:

—

Poured-plate method using de Man, Rogosa and Sharpe (MRS) agar ISO 15214

For the enumeration of Lactobacilli in the feed additive and feedingstuffs:

—

Poured-plate method using MRS agar EN 15787

For the enumeration of Saccharomyces cerevisiae PCM KKP 2059p in the feed additive and feedingstuffs:

—

Poured-plate method using chloramphenicol glucose yeast extract (CGYE) agar EN 15789

For the identification of Lactobacilli, Lactococcus lactis PCM B/00039 and Carnobacterium divergens PCM KKP 2012p:

—

Identification: Pulsed-Field Gel Electrophoresis (PFGE)

For the identification of Saccharomyces cerevisiae PCM KKP 2059p:

—

Polymerase Chain Reaction (PCR)

Chickens for fattening

—

5 × 10 8 (LAB)

5 × 10 6 ( Saccharomyces cerevisiae PCM KKP 2059p)

—

1.

In the directions for use of the additive and premixtures, indicate the storage temperature, storage life and stability to pelleting.

2.

The use is permitted in feed containing the following authorised coccidiostats: narasin/nicarbazin, salinomycin sodium, diclazuril, decoquinate, or maduramicin ammonium.

3.

For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including breathing and skin protection.

6 July 2027

( 1 )   Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports