Commission Implementing Regulation (EU) 2018/1090 of 31 July 2018 concerning the authorisation of a preparation of endo-1,4-beta-xylanase and endo-1,3(4)-beta-glucanase produced by Komagataella pastoris (CBS 25376) and Komagataella pastoris (CBS 26469) as a feed additive for chickens for fattening, chickens reared for laying, turkeys for fattening, all avian species reared for laying or for breeding purposes, weaned piglets and minor porcine species (weaned) (holder of the authorisation Kaesler Nutrition GmbH) (Text with EEA relevance.)

• CELEX: Implementing Regulation (EU) 2018/1090
• Articles: 3

Article 1

The preparation specified in the Annex, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘digestibility enhancers’, is authorised as an additive in animal nutrition, subject to the conditions laid down in that Annex.

Article 2

This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union .

Schedules & Appendices

ANNEX

ANNEX

Identification number of the additive

Name of the holder of authorisation

Additive

Composition, chemical formula, description, analytical method

Species or category of animal

Maximum age

Minimum content

Maximum content

Other provisions

End of period of authorisation

Units of activity/kg of complete feedingstuff with a moisture content of 12 %

Category of zootechnical additives. Functional group: digestibility enhancers

4a 28

Kaesler Nutrition GmbH

Endo-1,4-beta-xylanase

EC 3.2.1.8

Endo-1,3(4)-beta-glucanase

EC 3.2.1.6

Additive composition

Preparation of endo-1,4-beta-xylanase and endo-1,3(4)-beta-glucanase produced by Komagataella pastoris (CBS 25376) and Komagataella pastoris (CBS 26469) having a minimum activity of:

25 000 LXU  ( 1 ) /g and 2 200 LGU  ( 2 ) /g

Solid and liquid form.

Characterisation of the active substance

endo-1,4-beta-xylanase and endo-1,3(4)-beta-glucanase produced by Komagataella pastoris (CBS 25376) and Komagataella pastoris (CBS 26469)

Analytical method

( 3 )

Determination in the feed additive, premixtures and feedingstuffs of xylanase

—

colorimetric method based on the quantification of water soluble dyed fragments produced by the action of endo-1,4-β-xylanase on cross-linked wheat arabinoxylan.

Determination in the feed additive, premixtures and feedingstuffs of endo-1,3(4)-beta-glucanase:

—

colorimetric method based on the quantification of water soluble dyed fragments produced by the action of endo-1,3(4)-beta-glucanase on cross-linked azo-barley-glucan.

Chickens for fattening

Chickens reared for laying

—

4 250 LXU

375 LGU

—

1.

In the directions for use of the additive and premixtures, the storage conditions and stability to heat treatment shall be indicated.

2.

For use in weaned piglets up to 35 kg of body weight.

3.

For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks from their use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment.

21.8.2028

Turkeys for fattening

All avian species reared for laying or for breeding purposes other than chickens reared for laying

1 400 LXU

120 LGU

Piglets (weaned)

Minor porcine species (weaned)

700 LXU

60 LGU

( 1 )   1 LXU is the amount of enzyme which releases one micromole of reducing sugars equivalents (as xylose) from birch xylan per minute at pH 5,5 and 50 °C.

( 2 )   1 LGU is the amount of enzyme which releases one micromole of reducing sugars equivalents (as glucose) from barley glucan per minute at pH 5,5 and 50 °C.

( 3 )   Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports