Commission Implementing Regulation (EU) 2024/251 of 16 January 2024 concerning the renewal of the authorisation of the preparations of Lactiplantibacillus plantarum CNCM I-3235, Lactiplantibacillus plantarum DSM 11672/CNCM I-3736, Pediococcus acidilactici CNCM I-3237, Pediococcus acidilactici DSM 11673/CNCM I-4622, Pediococcus pentosaceus NCIMB 12455, Acidipropionibacterium acidipropionici CNCM I-4661, Lentilactobacillus buchneri NCIMB 40788/CNCM I-4323 and Lentilactobacillus hilgardii CNCM I-4785 and Lentilactobacillus buchneri CNCM I-4323/NCIMB 40788 as feed additives for all animal species, amending Implementing Regulations (EU) No 1065/2012, (EU) No 1119/2012, (EU) No 1113/2013 and (EU) No 304/2014 and repealing Implementing Regulations (EU) No 990/2012 and (EU) 2019/764
Data as of 2026-07-04 · Compiled from an official source version. Later amendments or repeals may not be reflected; the official text prevails. · Read the official text ↗
Renewal of authorisation
The authorisation of the preparations specified in the Annex, belonging to the additive category ‘technological additives’ and to the functional group ‘silage additives’, are renewed subject to the conditions laid down in that Annex.
Amendment to Implementing Regulation (EU) No 1065/2012
In the Annex to Implementing Regulation (EU) No 1065/2012, entry 1k20717 on Lactobacillus plantarum (CNCM I-3235) and entry 1k20722 on Lactobacillus plantarum (CNCM MA 18/5U) are deleted.
Amendment to Implementing Regulation (EU) No 1119/2012
In the Annex to Implementing Regulation (EU) No 1119/2012, entry 1k2104 on Pediococcus acidilactici CNCM MA 18/5M DSM 11673 and entry 1k2106 on Pediococcus pentosaceus NCIMB 12455 are deleted.
Amendment to Implementing Regulation (EU) No 1113/2013
In the Annex to Implementing Regulation (EU) No 1113/2013, entry 1k20739 on Lactobacillus buchneri NCIMB 40788/CNCM I-4323 is deleted.
Amendment to Implementing Regulation (EU) No 304/2014
In the Annex to Implementing Regulation (EU) No 304/2014, entry 1k21009 on Pediococcus acidilactici CNCM I-3237 is deleted.
Repeal of Implementing Regulations (EU) No 990/2012 and (EU) 2019/764
Implementing Regulations (EU) No 990/2012 and (EU) 2019/764 are repealed.
Transitional measures
The preparations of Lactiplantibacillus plantarum CNCM I-3235, Lactiplantibacillus plantarum DSM 11672/CNCM I-3736, Pediococcus acidilactici DSM 11673/CNCM I-4622, Acidipropionibacterium acidipropionici CNCM I-4661, Lentilactobacillus buchneri NCIMB 40788/CNCM I-4323 and Lentilactobacillus hilgardii CNCM I-4785 and Lentilactobacillus buchneri CNCM I-4323/NCIMB 40788 specified in the Annex, and feed containing them which are produced and labelled before 6 February 2025 in accordance with the rules applicable before 6 February 2024 may continue to be placed on the market and used until the existing stocks are exhausted.
Entry into force
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
ANNEX Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU/kg of fresh material Category of technological additives. Functional group: silage additives 1k20717 Lactiplantibacillus plantarum CNCM I-3235 Additive composition Preparation of Lactiplantibacillus plantarum CNCM I-3235 containing a minimum of 5 × 10 10 CFU/g additive Characterisation of the active substance Viable cells of Lactiplantibacillus plantarum CNCM I-3235 Analytical method ( 1 ) — Enumeration: Spread plate method: EN 15787 — Identification: Pulsed- Field Gel Electrophoresis (PFGE) – CEN/TS 17697 or DNA sequencing methods All animal species — — — 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other microorganisms as silage additives: 2 × 10 7 CFU/kg fresh material. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal skin, eye and breathing protective equipment. 6 February 2034 Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU/kg of fresh material Category of technological additives. Functional group: silage additives 1k20722 Lactiplantibacillus plantarum DSM 11672/CNCM I-3736 Additive composition Preparation of Lactiplantibacillus plantarum DSM 11672/CNCM I-3736 containing a minimum of 2 × 10 10 CFU/g additive Characterisation of the active substance Viable cells of Lactiplantibacillus plantarum DSM 11672/CNCM I-3736 Analytical method ( 2 ) — Enumeration: Spread plate method: EN 15787 — Identification: Pulsed- Field Gel Electrophoresis (PFGE) – CEN/TS 17697 or DNA sequencing methods All animal species — — — 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other microorganisms as silage additives: 1 × 10 8 CFU/kg fresh material. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal skin, eye and breathing protective equipment. 6 February 2034 Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU/kg of fresh material Category of technological additives. Functional group: silage additives 1k21009 Pediococcus acidilactici CNCM I-3237 Additive composition Preparation of Pediococcus acidilactici CNCM I-3237 containing a minimum of 1 × 10 10 CFU/g additive Characterisation of the active substance Viable cells of Pediococcus acidilactici CNCM I-3237 Analytical method ( 3 ) — Enumeration: Spread plate method: EN 15786 — Identification: Pulsed- Field Gel Electrophoresis (PFGE) – CEN/TS 17697 or DNA sequencing methods All animal species — — — 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other microorganisms as silage additives: 5 × 10 7 CFU/kg fresh material. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal skin, eye and breathing protective equipment 6 February 2034 1k2104 Pediococcus acidilactici DSM 11673/CNCM I-4622 Additive composition Preparation of Pediococcus acidilactici DSM 11673/CNCM I-4622 containing a minimum of 3 × 10 9 CFU/g additive. Characterisation of the active substance Viable cells of Pediococcus. acidilactici DSM 11673/CNCM I-4622 Analytical method ( 3 ) — Enumeration: Spread plate method: EN 15786 — Identification: Pulsed- Field Gel Electrophoresis (PFGE) – CEN/TS 17697 or DNA sequencing methods All animal species — — — 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other microorganisms as silage additives: 3 × 10 7 CFU/kg fresh material. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal skin and breathing protective equipment. 6 February 2034 Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU/kg of fresh material Category of technological additives. Functional group: silage additives 1k2106 Pediococcus pentosaceus NCIMB 12455 Additive composition Preparation of Pediococcus pentosaceus NCIMB 12455 containing a minimum of 3 × 10 9 CFU/g additive Characterisation of the active substance Viable cells of Pediococcus pentosaceus NCIMB 12455 Analytical method ( 4 ) — Enumeration: Spread plate method: EN 15786 — Identification: Pulsed- Field Gel Electrophoresis (PFGE) – CEN/TS 17697 or DNA sequencing methods All animal species — — — 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other microorganisms as silage additives: 3 × 10 7 CFU/kg fresh material. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal skin, eye and breathing protective equipment. 6 February 2034 Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU/kg of fresh material Category of technological additives. Functional group: silage additives 1k2111 Acidipropionibacterium acidipropionici CNCM I-4661 Additive composition Preparation of Acidipropionibacterium acidipropionici CNCM I-4661 containing a minimum of 1 × 10 8 CFU/g additive Characterisation of the active substance Viable cells of Acidipropionibacterium acidipropionici CNCM I-4661 Analytical method ( 5 ) — Enumeration: Spread plate method: EN 15787 — Identification: Pulsed- Field Gel Electrophoresis (PFGE) – CEN/TS 17697 or DNA sequencing methods All animal species — — — 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other microorganisms as silage additives: 1 × 10 8 CFU/kg fresh material. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal skin, eye and breathing protective equipment. 6 February 2034 Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU/kg of fresh material Category of technological additives. Functional group: silage additives 1k20739 Lentilactobacillus. buchneri NCIMB 40788/CNCM I-4323 Additive composition Preparation of Lentilactobacillus buchneri NCIMB 40788/CNCM I-4323 containing a minimum of 3 × 10 9 CFU/g additive Characterisation of the active substance Viable cells of Lentilactobacillus buchneri NCIMB 40788/CNCM I-4323 Analytical method ( 6 ) — Enumeration: Spread plate method: EN 15787 — Identification: Pulsed- Field Gel Electrophoresis (PFGE) – CEN/TS 17697 or DNA sequencing methods All animal species — — — 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 8 CFU/kg fresh material. 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal skin, eye and breathing protective equipment. 6 February 2034 Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU/kg of fresh material Category of technological additives. Functional group: silage additives 1k20757 Lentilactobacillus hilgardii CNCM I-4785 and Lentilactobacillus. buchneri CNCM I-4323/NCIMB 40788 Additive composition Preparation of Lentilactobacillus hilgardii CNCM I-4785 and Lentilactobacillus buchneri CNCM I-4323/NCIMB 40788 containing a minimum of 1,5 × 10 11 CFU/g additive (ratio of 1:1) Characterisation of the active substance Viable cells of Lentilactobacillus hilgardii CNCM I-4785 and Lentilactobacillus buchneri CNCM I-4323/NCIMB 40788 Analytical method ( 7 ) — Enumeration: Spread plate method: EN 15787 — Identification: Pulsed- Field Gel Electrophoresis (PFGE) – CEN/TS 17697 or DNA sequencing methods All animal species — — — 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other micro-organisms as silage additives: 3 × 10 8 CFU/kg ( L. hilgardii CNCM I-4785 and L. buchneri CNCM I-4323/NCIMB 40788 in ratio of 1:1) of easy and moderately difficult to ensile fresh material ( 8 ) . 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from their use. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with personal skin, eye and breathing protective equipment. 6 February 2034 ( 1 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en ( 2 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en ( 3 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en ( 4 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en ( 5 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en ( 6 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en ( 7 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en ( 8 ) Easy to ensile forage: > 3 % soluble carbohydrates in fresh material. Moderately difficult to ensile forage: 1,5–3,0 % soluble carbohydrates in the fresh material. Commission Regulation (EC) No 429/2008 of 25 April 2008 on detailed rules for the implementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of applications and the assessment and the authorisation of feed additives ( OJ L 133, 22.5.2008, p. 1 ).
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