Article 1
Open ↗Annex I to Implementing Regulation (EU) 2015/1375 is amended as follows: (1) Chapter I is replaced by the following: ‘CHAPTER I REFERENCE METHOD OF DETECTION 1. The reference method of detection for the examination of samples for Trichinella , shall be ISO 18743:2015/Amd1:2023. 2. The following rules shall apply only to the examination of meat of domestic swine: (a) In the case of whole carcasses of domestic swine other than breeding sows and boars, a specimen weighing at least 1 g shall be taken from a pillar of the diaphragm at the transition to the sinewy part. Special trichinae forceps may be used provided that an accuracy of between 1,00 and 1,15 g can be guaranteed. In the case of whole carcasses of breeding sows and boars, a larger sample weighing at least 2 g shall be taken from a pillar of the diaphragm at the transition to the sinewy part. In the absence of diaphragm pillars, a specimen of 2 g shall be taken from the rib part or the breastbone part of the diaphragm, or from the jaw muscle, tongue or abdominal muscles of domestic swine other than breeding sows and boars, and a specimen of 4 g shall be taken from the same tissues of breeding sows and boars. (b) For cuts of meat, a sample weighing at least 5 g of striated muscle, and containing little fat shall be taken, where possible from close to bones or tendons. A sample of the same size shall be collected from meat that is not intended to be cooked thoroughly or other types of post-slaughter processing.’; (2) Chapter II is amended as follows: (a) the wording ‘ISO 18743:2015’ is replaced by ‘ISO 18743:2015/Amd1:2023’ in the following provisions: (i) Part A, point 1(o) and (q), second paragraph; (ii) Part A, point 3.IV, first and final sentences; (iii) Part C, point 1(f), second paragraph; (iv) Part D, point 1(m), second paragraph; (v) Part D, point 3.II, heading; and (vi) Part D, point 3.III, first and third paragraphs; (b) the wording in Part A, point 2, Part B, point 2, Part C, point 2, and Part D, point 2 is replaced by the following: ‘2. Collecting of specimens and quantity to be digested As stipulated in Chapter I and in point 4.2 of the ISO 18743:2015/Amd1:2023 (see also Annexes A and B thereto for further details).’; (c) the following Part F is added: ‘F. Automated detection of Trichinella spp. using the lumiVAST Trichinella method This method shall only be considered equivalent for the testing of meat of domestic swine Sample preparation and analysis shall be carried out in accordance with the instructions provided by the manufacturer. All apparatuses used for this method shall be calibrated and maintained in accordance with the instructions provided by the manufacturer(s). Where examination of a collective sample produces a positive or uncertain result, a further 20 g sample shall be taken from a pillar of the diaphragm or other tissue of each swine in accordance with Chapter I. The 20 g samples from 5 swine shall be pooled and examined using the same method. When a positive result is obtained from a group of 5 swine, a further 20 g sample shall be collected from the individuals in the group and each shall be examined separately using the same method. When a positive or uncertain result is obtained, at least 20 g of swine muscle shall be sent to the national reference laboratory for confirmation using the reference method laid down in Chapter I. Parasite samples shall be kept in 70 to 90 % ethyl alcohol for conservation and identification at species level at the EU or national reference laboratory.’.