ANNEX IVSupplementary provisions
ANNEX IV In the Annex to Regulation (EC) No 833/2005, the entry for E 1627 is replaced by the following: ‘E 1627 Endo-1,3(4)-beta-glucanase EC 3.2.1.6 Endo-1,4-beta-xylanase EC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of: powder form: endo-1,3(4)-beta-glucanase: 800 U ( 1 ) /g endo-1,4-beta-xylanase: 800 U ( 2 ) /g liquid form: endo-1,3(4)-beta-glucanase: 800 U/ml endo-1,4-beta-xylanase: 800 U/ml Pigs for fattening — endo-1,3(4)-beta-glucanase: 400 U — 1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting. 2. Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta-glucanase: 400 U endo-1,4-beta-xylanase: 400 U. 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans) e.g. containing more than 65 % barley. Without a time-limit endo-1,4-beta-xylanase: 400 U — ( 1 ) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C. ( 2 ) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.’