Product
Article 2
1. The genetically modified organisms to be placed on the market as product, hereinafter ‘the product’, are cut flowers of carnation ( Dianthus caryophyllus L.), with modified flower colour, derived from the Dianthus caryophyllus L. cell culture, and transformed with Agrobacterium tumefaciens , strain AGL0, using the vector pCGP1991 and resulting in line 123.8.12. The product contains the following DNA in three cassettes: (a) Cassette 1 The petunia dfr gene from Petunia X Hybrida encoding dihydroflavonol 4-reductase (DFR), a key enzyme in the anthocyanin biosynthetic pathway. The dfr gene is under control of its own promoter and terminator. (b) Cassette 2 The promoter from a snapdragon gene encoding chalcone synthase, petunia flavonoid 3’5’ hydroxylase (F3’5’H) cDNA, a key enzyme in the anthocyanin biosynthetic pathway, the terminator from the petunia gene encoding a phospholipid transfer protein homologue. Simultaneous expression of both dfr and f3’5’h genes in carnation results in a modified flavonoid synthesis in flowers, and subsequent formation of the blue pigment delphinidin. (c) Cassette 3 The cauliflower mosaic virus 35S promoter, a non-translated region from the cDNA corresponding to the petunia gene encoding chlorophyll a/b binding protein 5, the SuRB ( als ) gene coding for a mutant acetolactate synthase protein (ALS), which confers tolerance to sulfonylurea, derived from Nicotiana tabacum , including its terminator. This gene was used for in vitro selection. 2. The consent shall cover progeny derived through vegetative reproduction of the genetically modified carnation ( Dianthus caryophyllus L., line 123.8.12).