ANNEXSupplementary provisions
ANNEX Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU/kg of fresh material Category of technological additives. Functional group: silage additives 1k20716 — Lactobacillus plantarum (DSM 23375) Additive composition Preparation of Lactobacillus plantarum (DSM 23375) containing a minimum of 2 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (DSM 23375) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 8 CFU/kg fresh material. 3. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20717 — Lactobacillus plantarum (CNCM I-3235) Additive composition Preparation of Lactobacillus plantarum (CNCM I-3235) containing a minimum of 5 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (CNCM I-3235) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 2 × 10 7 CFU/kg fresh material. 3. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20718 — Lactobacillus plantarum (DSM 19457) Additive composition Preparation of Lactobacillus plantarum (DSM 19457) containing a minimum of 1 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (DSM 19457) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 5 × 10 7 CFU/kg fresh material. 3. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20719 — Lactobacillus plantarum (DSM 16565) Additive composition Preparation of Lactobacillus plantarum (DSM 16565) containing a minimum of 5 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (DSM 16565) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 8 CFU/kg fresh material. 3. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20720 — Lactobacillus plantarum (DSM 16568) Additive composition Preparation of Lactobacillus plantarum (DSM 16568) containing a minimum of 5 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (DSM 16568) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 8 CFU/kg fresh material. 3. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20721 — Lactobacillus plantarum (LMG 21295) Additive composition Preparation of Lactobacillus plantarum (LMG 21295) containing a minimum of 5 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (LMG 21295) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 8 CFU/kg fresh material. 3. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20722 — Lactobacillus plantarum (CNCM MA 18/5U) Additive composition Preparation of Lactobacillus plantarum (CNCM MA 18/5U) containing a minimum of 2 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (CNCM MA 18/5U) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 8 CFU/kg fresh material. 3. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20723 — Lactobacillus plantarum (NCIMB 30094) Additive composition Preparation of Lactobacillus plantarum (NCIMB 30094) containing a minimum of 5 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (NCIMB 30094) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 9 CFU/kg fresh material. 3. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20724 — Lactobacillus plantarum (VTT E-78076) Additive composition Preparation of Lactobacillus plantarum (VTT E-78076) containing a minimum of 1 × 10 11 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (VTT E-78076) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 9 CFU/Kg fresh material. 3. The additive shall be used in easy and moderately difficult to ensile material ( 2 ) . 4. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20725 — Lactobacillus plantarum (ATCC PTSA-6139) Additive composition Preparation of Lactobacillus plantarum (ATCC PTSA-6139) containing a minimum of 1 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (ATCC PTSA-6139) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 2 × 10 7 CFU/kg fresh material. 3. The additive shall be used in easy to ensile material ( 3 ) . 4. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20726 — Lactobacillus plantarum (DSM 18112) Additive composition Preparation of Lactobacillus plantarum (DSM 18112) containing a minimum of 1 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (DSM 18112) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 5 × 10 6 CFU/kg fresh material. 3. The additive shall be used in easy to ensile material ( 3 ) . 4. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20727 — Lactobacillus plantarum (DSM 18113) Additive composition Preparation of Lactobacillus plantarum (DSM 18113) containing a minimum of 1 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (DSM 18113) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 2 × 10 7 CFU/kg fresh material. 3. The additive shall be used in easy to ensile material ( 3 ) . 4. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20728 — Lactobacillus plantarum (DSM 18114) Additive composition Preparation of Lactobacillus plantarum (DSM 18114) containing a minimum of 1 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (DSM 18114) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 2 × 10 7 CFU/kg fresh material. 3. The additive shall be used in easy to ensile material ( 3 ) . 4. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20729 — Lactobacillus plantarum (ATCC 55943) Additive composition Preparation of Lactobacillus plantarum (ATCC 55943) containing a minimum of 1 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (ATCC 55943) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 2 × 10 7 CFU/kg fresh material. 3. The additive shall be used in easy to ensile material ( 3 ) . 4. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 1k20730 — Lactobacillus plantarum (ATCC 55944) Additive composition Preparation of Lactobacillus plantarum (ATCC 55944) containing a minimum of 1 × 10 10 CFU/g additive Characterisation of the active substance Lactobacillus plantarum (ATCC 55944) Analytical method ( 1 ) Enumeration in the feed additive: spread plate method using MRS agar (EN 15787) Identification in the feed additive: pulsed-field gel electrophoresis (PFGE). All animal species — — — 1. In the directions for use of the additive and premixture, indicate the storage temperature and storage life. 2. Minimum dose of the additive when used without combination with other micro-organisms as silage additives: 5 × 10 6 CFU/kg fresh material. 3. The additive shall be used in easy to ensile material ( 3 ) . 4. For safety: it is recommended to use breathing protection and gloves during handling. 4 December 2022 ( 1 ) Details of the analytical methods are available at the following address of the Reference Laboratory: http://irmm.jrc.ec.europa.eu/EURLs/EURL_feed_additives/Pages/index.aspx ( 2 ) Easy to ensile forage: > 3 % soluble carbohydrates in fresh material (e.g. whole plant maize, ryegrass, brome grass or sugar beet pulp). Moderately difficult to ensile forages: 1,5-3,0 % soluble carbohydrate in fresh material (e.g. meadow grass, fescue or wilted alfalfa). Commission Regulation (EC) No 429/2008 ( OJ L 133, 22.5.2008, p. 1 ). ( 3 ) Easy to ensile forage: > 3 % soluble carbohydrates in fresh material (e.g. whole plant maize, ryegrass, brome grass or sugar beet pulp). Regulation (EC) No 429/2008.