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Article 2
1. The genetically modified organism to be placed on the market, is a carnation ( Dianthus caryophyllus L.) with modified flower colour, derived from a Dianthus caryophyllus L. cell culture, and transformed with Agrobacterium tumefaciens , strain AGL0, using the vector pCGP1991, and resulting in line SHD-27531-4. The genetically modified carnation contains the following DNA in three cassettes: (a) Cassette 1 The petunia dfr gene encoding dihydroflavonol 4-reductase (DFR), a key enzyme in the anthocyanin biosynthetic pathway, including its own promoter and terminator. (b) Cassette 2 The promoter sequence from snapdragon chalcone synthase gene, flavonoid 3′5′-hydroxylase ( f3′5′h ) from Viola hortensis cDNA encoding F3′5′H, a key enzyme in the anthocyanin biosynthetic pathway, and the terminator from the D8 petunia gene encoding a putative phospholipid transfer protein. These two cassettes were inserted into the plant genome to obtain the desired flower colour. (c) Cassette 3 The Cauliflower mosaic virus 35S promoter, the 5′-untranslated region from the petunia gene encoding chlorophyll a/b binding protein, the SuRB ( als ) gene coding for a mutant acetolactate synthase protein (ALS) derived from Nicotiana tabacum, which confers tolerance to sulfonylurea, including its own terminator. This trait was used as a marker in the selection of transformants. 2. The unique identifier of the genetically modified carnation shall be SHD-27531-4.