ANNEXSupplementary provisions
ANNEX Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation Units of activity/kg of complete feedingstuff with a moisture content of 12 % Category of zootechnical additives. Functional group: digestibility enhancers 4a23 Lohmann Animal Nutrition GmbH 6-phytase EC 3.1.3.26 Additive composition Preparation of 6-phytase produced by Komagataella pastoris (DSM 25375) having a minimum activity of: 40 000 U ( 1 ) /g Liquid and solid form Characterisation of the active substance 6-phytase (EC 3.1.3.26) produced by Komagataella pastoris (DSM 25375) Analytical method ( 2 ) For the quantification of 6-phytase activity in the feed additive: colorimetric method based on the enzymatic reaction of phytase on the phytate — VDLUFA Method Book, Vol. III, 27.1.1 For the quantification of 6-phytase activity in premixtures and mineral feeds: colorimetric method based on the enzymatic reaction of phytase on the phytate — VDLUFA Method Book, Vol. III, 27.1.3 For the quantification of 6-phytase activity in feedingstuffs: colorimetric method based on the enzymatic reaction of phytase on the phytate — EN ISO 30024. Chickens for fattening and reared for laying, all avian species for fattening and reared for laying other than turkeys for fattening and reared for breeding — 250 U — 1. In the directions for use of the additive and premixture, indicate the storage conditions and stability to heating treatment. 2. For safety: breathing protection, safety glasses and gloves shall be used during handling. 29 March 2026 All avian species for laying 125 U Turkeys for fattening and reared for breeding 500 U Piglets (weaned), pigs for fattening, sows and minor porcine species 250 U ( 1 ) 1 U is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute from a sodium phytate substrate at pH 5,5 and 37 °C. ( 2 ) Details of the analytical methods are available at the following address of the Reference Laboratory: http://irmm.jrc.ec.europa.eu/EURLs/EURL_feed_additives/Pages/index.aspx