ANNEX ISupplementary provisions
ANNEX I Requirements for the pathogen-specific surveillance programme provided for in Articles 2(3)(c), 4(2)(c) and Article 4(3)(c)(i) 1. The pathogen-specific surveillance programme, using appropriate risk-based or representative sampling, shall be designed to detect per epidemiologically relevant geographical unit in the Member State or part thereof the Echinococcus multilocularis parasite in the wild definitive host population, if present in any part of the Member State at a prevalence of not more than 1 % at a confidence level of at least 95 %. 2. The pathogen-specific surveillance programme shall describe the target wild definitive host population, including the density, age structure, geographical and gender distribution, taking into account the relative risks of infection with the Echinococcus multilocularis parasite in different species and subpopulations of the target wild definitive host population. 3. The pathogen-specific surveillance programme shall consist in the ongoing collection, during the 12-month surveillance period, of samples from wild definitive hosts to be analysed using: (a) the sedimentation and counting technique (SCT), or a technique of equivalent sensitivity and specificity, by examination of intestinal contents for the detection of the Echinococcus multilocularis parasite; or (b) polymerase chain reaction (PCR) methods, or a technique of equivalent sensitivity and specificity, by examination of intestinal contents or faeces for the detection of species-specific deoxyribonucleic acid (DNA) from tissue or eggs of the Echinococcus multilocularis parasite.