Article 1
The preparation belonging to the group ‘Enzymes’, as specified in Annex I, is authorised without a time limit as additive in animal nutrition under the conditions laid down in that Annex.
Commission Regulation (EU) No 1270/2009 of 21 December 2009 concerning the permanent authorisations of certain additives in feedingstuffs (Text with EEA relevance)
Commission Regulation (EU) No 1270/2009 of 21 December 2009 concerning the permanent authorisations of certain additives in feedingstuffs (Text with EEA relevance)
Data as of 2026-07-04 · Compiled from an official source version. Later amendments or repeals may not be reflected; the official text prevails. · Read the official text ↗
Article 2
The preparation belonging to the group ‘Enzymes’, as specified in Annex II, is authorised without a time limit as additive in animal nutrition under the conditions laid down in that Annex.
Article 3
This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union .
Supplementary provisions
ANNEX ISupplementary provisions
ANNEX I EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation Units of activity/kg of complete feedingstuff Enzymes E 1636 Endo-1,3(4)-beta-glucanase EC 3.2.1.6 Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of: Solid form: 700 000 BU ( 1 ) /g Liquid form: 300 000 BU/g Piglets (weaned) — 17 500 BU — 1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting. 2. For use in weaned piglets up to 35 kg. 3. Recommended dose per kilogram of complete feedingstuff: 17 500 -50 000 BU. 4. For use in compound feed rich in non-starch polysaccharides (mainly glucans), e.g. containing more than 60 % barley or wheat. Without a time limit ( 1 ) 1 BU is the amount of enzyme which liberates 0,06 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,8 and 50 °C.
ANNEX IISupplementary provisions
ANNEX II EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation Units of activity/kg of complete feedingstuff Enzymes E 1620 Endo-1,3(4)-beta-glucanase EC 3.2.1.6 Endo-1,4-beta-glucanase EC 3.2.1.4 Alpha-amylase EC 3.2.1.1 Bacillolysin EC 3.4.24.28 Endo-1,4-beta-xylanase EC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) and endo-1,4-beta-xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of: Endo-1,3(4)-beta-glucanase: 2 350 U/g ( 1 ) Endo-1,4-beta-glucanase: 4 000 U/g ( 2 ) Alpha-amylase: 400 U/g ( 3 ) Bacillolysin: 450 U/g ( 4 ) Endo-1,4-beta-xylanase: 20 000 U/g ( 5 ) Turkeys for fattening — Endo-1,3(4)-beta-glucanase: 587 U — 1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kilogram of complete feedingstuff: — endo-1,3(4)-beta-glucanase: 1 175 -2 350 U, — endo-1,4-beta-glucanase: 2 000 -4 000 U, — alpha-amylase: 200-400 U, — bacillolysin: 225-450 U, — endo-1,4-beta-xylanase: 10 000 -20 000 U, 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and especially arabinoxylans), e.g. containing more than 30 % wheat. Without a time limit Endo-1,4-beta-glucanase: 1 000 U — Alpha-amylase: 100 U — Bacillolysin: 112 U — Endo-1,4-beta-xylanase: 5 000 U — Piglets (weaned) — Endo-1,3(4)-beta-glucanase: 1 175 U — 1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. For use in weaned piglets up to 35 kg 3. Recommended dose per kilogram of complete feedingstuff: — endo-1,3(4)-beta-glucanase: 1 175 U, — endo-1,4-beta-glucanase: 2 000 U, — alpha-amylase: 200 U, — bacillolysin: 225 U, — endo-1,4-beta-xylanase: 10 000 U, 4. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and especially arabinoxylans), e.g. containing more than 35 % wheat. Endo-1,4-beta-glucanase: 2 000 U — Alpha-amylase: 200 U — Bacillolysin: 225 U — Endo-1,4-beta-xylanase: 10 000 U — ( 1 ) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30 °C. ( 2 ) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 4,8 and 50 °C. ( 3 ) 1 U is the amount of enzyme which liberates 1 micromole of glucose from a cross-linked starch polymer per minute at pH 7,5 and 37 °C. ( 4 ) 1 U is the amount of enzyme which solubilises one microgram of azo-casein substrate per minute at pH 7,5 and 37 °C. ( 5 ) 1 U is the amount of enzyme which liberates 0,0067 micromoles of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 5,3 and 50 °C.
Source: EUR-Lex (Publications Office of the EU), © European Union, reuse permitted under Commission Decision 2011/833/EU.
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