Commission Regulation (EU) No 1270/2009 of 21 December 2009 concerning the permanent authorisations of certain additives in feedingstuffs (Text with EEA relevance)

• CELEX: Regulation (EU) No 1270/2009
• Articles: 5

Article 1

The preparation belonging to the group ‘Enzymes’, as specified in Annex I, is authorised without a time limit as additive in animal nutrition under the conditions laid down in that Annex.

Article 2

The preparation belonging to the group ‘Enzymes’, as specified in Annex II, is authorised without a time limit as additive in animal nutrition under the conditions laid down in that Annex.

Article 3

This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union .

Schedules & Appendices

ANNEX I

ANNEX I

EC No

Additive

Chemical formula, description

Species or category of animal

Maximum age

Minimum content

Maximum content

Other provisions

End of period of authorisation

Units of activity/kg of complete feedingstuff

Enzymes

E 1636

Endo-1,3(4)-beta-glucanase

EC 3.2.1.6

Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of:

Solid form: 700 000  BU  ( 1 ) /g

Liquid form: 300 000  BU/g

Piglets (weaned)

—

17 500  BU

—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.

2.

For use in weaned piglets up to 35 kg.

3.

Recommended dose per kilogram of complete feedingstuff: 17 500 -50 000  BU.

4.

For use in compound feed rich in non-starch polysaccharides (mainly glucans), e.g. containing more than 60 % barley or wheat.

Without a time limit

( 1 )   1 BU is the amount of enzyme which liberates 0,06 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,8 and 50 °C.

ANNEX II

ANNEX II

EC No

Additive

Chemical formula, description

Species or category of animal

Maximum age

Minimum content

Maximum content

Other provisions

End of period of authorisation

Units of activity/kg of complete feedingstuff

Enzymes

E 1620

Endo-1,3(4)-beta-glucanase EC 3.2.1.6

Endo-1,4-beta-glucanase EC 3.2.1.4

Alpha-amylase EC 3.2.1.1

Bacillolysin EC 3.4.24.28

Endo-1,4-beta-xylanase EC 3.2.1.8

Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) and endo-1,4-beta-xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of:

Endo-1,3(4)-beta-glucanase: 2 350  U/g  ( 1 )

Endo-1,4-beta-glucanase: 4 000  U/g  ( 2 )

Alpha-amylase: 400 U/g  ( 3 )

Bacillolysin: 450 U/g  ( 4 )

Endo-1,4-beta-xylanase: 20 000  U/g  ( 5 )

Turkeys for fattening

—

Endo-1,3(4)-beta-glucanase: 587 U

—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2.

Recommended dose per kilogram of complete feedingstuff:

—

endo-1,3(4)-beta-glucanase: 1 175 -2 350  U,

—

endo-1,4-beta-glucanase: 2 000 -4 000  U,

—

alpha-amylase: 200-400 U,

—

bacillolysin: 225-450 U,

—

endo-1,4-beta-xylanase: 10 000 -20 000  U,

3.

For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and especially arabinoxylans), e.g. containing more than 30 % wheat.

Without a time limit

Endo-1,4-beta-glucanase: 1 000  U

—

Alpha-amylase: 100 U

—

Bacillolysin: 112 U

—

Endo-1,4-beta-xylanase: 5 000  U

—

Piglets (weaned)

—

Endo-1,3(4)-beta-glucanase: 1 175  U

—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2.

For use in weaned piglets up to 35 kg

3.

Recommended dose per kilogram of complete feedingstuff:

—

endo-1,3(4)-beta-glucanase: 1 175  U,

—

endo-1,4-beta-glucanase: 2 000  U,

—

alpha-amylase: 200 U,

—

bacillolysin: 225 U,

—

endo-1,4-beta-xylanase: 10 000  U,

4.

For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and especially arabinoxylans), e.g. containing more than 35 % wheat.

Endo-1,4-beta-glucanase: 2 000  U

—

Alpha-amylase: 200 U

—

Bacillolysin: 225 U

—

Endo-1,4-beta-xylanase: 10 000  U

—

( 1 )   1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30 °C.

( 2 )   1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 4,8 and 50 °C.

( 3 )   1 U is the amount of enzyme which liberates 1 micromole of glucose from a cross-linked starch polymer per minute at pH 7,5 and 37 °C.

( 4 )   1 U is the amount of enzyme which solubilises one microgram of azo-casein substrate per minute at pH 7,5 and 37 °C.

( 5 )   1 U is the amount of enzyme which liberates 0,0067 micromoles of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 5,3 and 50 °C.