ANNEX IISupplementary provisions
ANNEX II EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation Units of activity/kg of complete feedingstuff Enzymes E 1620 Endo-1,3(4)-beta-glucanase EC 3.2.1.6 Endo-1,4-beta-glucanase EC 3.2.1.4 Alpha-amylase EC 3.2.1.1 Bacillolysin EC 3.4.24.28 Endo-1,4-beta-xylanase EC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), bacillolysin produced by Bacillus amyloliquefaciens (DSM 9554) and endo-1,4-beta-xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of: Endo-1,3(4)-beta-glucanase: 2 350 U/g ( 1 ) Endo-1,4-beta-glucanase: 4 000 U/g ( 2 ) Alpha-amylase: 400 U/g ( 3 ) Bacillolysin: 450 U/g ( 4 ) Endo-1,4-beta-xylanase: 20 000 U/g ( 5 ) Turkeys for fattening — Endo-1,3(4)-beta-glucanase: 587 U — 1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Recommended dose per kilogram of complete feedingstuff: — endo-1,3(4)-beta-glucanase: 1 175 -2 350 U, — endo-1,4-beta-glucanase: 2 000 -4 000 U, — alpha-amylase: 200-400 U, — bacillolysin: 225-450 U, — endo-1,4-beta-xylanase: 10 000 -20 000 U, 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and especially arabinoxylans), e.g. containing more than 30 % wheat. Without a time limit Endo-1,4-beta-glucanase: 1 000 U — Alpha-amylase: 100 U — Bacillolysin: 112 U — Endo-1,4-beta-xylanase: 5 000 U — Piglets (weaned) — Endo-1,3(4)-beta-glucanase: 1 175 U — 1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. For use in weaned piglets up to 35 kg 3. Recommended dose per kilogram of complete feedingstuff: — endo-1,3(4)-beta-glucanase: 1 175 U, — endo-1,4-beta-glucanase: 2 000 U, — alpha-amylase: 200 U, — bacillolysin: 225 U, — endo-1,4-beta-xylanase: 10 000 U, 4. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and especially arabinoxylans), e.g. containing more than 35 % wheat. Endo-1,4-beta-glucanase: 2 000 U — Alpha-amylase: 200 U — Bacillolysin: 225 U — Endo-1,4-beta-xylanase: 10 000 U — ( 1 ) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30 °C. ( 2 ) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 4,8 and 50 °C. ( 3 ) 1 U is the amount of enzyme which liberates 1 micromole of glucose from a cross-linked starch polymer per minute at pH 7,5 and 37 °C. ( 4 ) 1 U is the amount of enzyme which solubilises one microgram of azo-casein substrate per minute at pH 7,5 and 37 °C. ( 5 ) 1 U is the amount of enzyme which liberates 0,0067 micromoles of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 5,3 and 50 °C.