The substance specified in the Annex, belonging to the additive category ‘nutritional additives’ and to the functional group ‘amino acids, their salts and analogues’, is authorised as an additive in animal nutrition, subject to the conditions laid down in that Annex.
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.
ANNEXSupplementary provisions
ANNEX
Identification number of the additive
Name of the holder of authorisation
Additive
Composition, chemical formula, description, analytical method.
Species or category of animal
Maximum age
Minimum content
Maximum content
Other provisions
End of period of authorisation
mg/kg of complete feed with a moisture content of 12 %
Category of nutritional additives. Functional group: amino acids, their salts and analogues.
3c410
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L-threonine
Additive composition
Powder with a minimum of 98 %
L-threonine (on a dry matter basis).
Characterisation of the active substance
L-threonine produced by fermentation with Corynebacterium glutamicum KCCM 80117 or
Corynebacterium glutamicum KCCM 80118
Chemical formula: C 4 H 9 NO 3
CAS Number: 72-19-5.
Analytical methods
( 1 )
For the determination of L-threonine in the feed additive:
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Food Chemical Codex ‘L-threonine monograph’, and
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Ion exchange chromatography coupled with post-column derivatisation and optical detection (IEC-VIS/FLD) – EN ISO 17180.
For the determination of threonine in premixtures:
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ion exchange chromatography coupled with post-column derivatisation and optical detection (IEC-VIS/FLD) – EN ISO 17180, and
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ion exchange chromatography coupled with post-column derivatisation and photometric detection (IEC-VIS), Commission Regulation (EC) No 152/2009 (Annex III, F).
For the determination of threonine in compound feed and feed materials:
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Ion exchange chromatography coupled with post-column derivatisation and photometric detection (IEC-VIS): Commission Regulation (EC) No 152/2009 (Annex III, F).
For the determination of threonine in water:
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ion exchange chromatography coupled with post-column derivatisation and optical detection (IEC-VIS/FLD).
All species
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1.
L-threonine may be placed on the market and used as an additive consisting of a preparation.
2.
L-threonine may be used via water for drinking.
3.
The labelling of the additive shall indicate the moisture content.
4.
The labelling of the additive and premixtures shall indicate the following:
‘The supplementation with L-threonine, in particular via water for drinking, should take into account all essential and conditional essential amino acids in order to avoid imbalances.’
12.3.2030
( 1 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports