Commission Implementing Regulation (EU) 2022/273 of 23 February 2022 concerning the authorisation of preparations of Lacticaseibacillus rhamnosus IMI 507023, Pediococcus pentosaceus IMI 507024, Pediococcus pentosaceus IMI 507025, Lactiplantibacillus plantarum IMI 507026, Lactiplantibacillus plantarum IMI 507027 and Lactiplantibacillus plantarum IMI 507028 as silage additives for all animal species (Text with EEA relevance)
Data as of 2026-07-04 · Compiled from an official source version. Later amendments or repeals may not be reflected; the official text prevails. · Read the official text ↗
The preparations specified in the Annex, belonging to the additive category ‘technological additives’ and to the functional group ‘silage additives’, are authorised as additives in animal nutrition, subject to the conditions laid down in that Annex.
This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union .
ANNEX Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU of additive/kg of fresh material Category of technological additives. Functional group: silage additives 1k21701 Lacticaseibacillus rhamnosus IMI 507023 Additive composition: Preparation of Lacticaseibacillus rhamnosus IMI 507023 containing a minimum of 1 × 10 10 CFU/g additive. Solid form Characterisation of the active substance: Viable cells of Lacticaseibacillus rhamnosus IMI 507023. - Analytical method ( 1 ) — Identification: Pulsed-Field Gel Electrophoresis (PFGE) — Enumeration in the feed additives: Spread plate method on MRS agar (EN 15787) All animal species - - - 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 9 CFU/kg of easy and moderately difficult to ensile fresh material ( 2 ) . 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin, eyes and breathing protection. 16.3.2032 Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU of additive/kg of fresh material Category of technological additives. Functional group: silage additives 1k21016 Pediococcus pentosaceus IMI 507024 Additive composition: Preparation of Pediococcus pentosaceus IMI 507024 containing a minimum of 1 × 10 10 CFU/g additive. Solid form Characterisation of the active substance: Viable cells of Pediococcus pentosaceus IMI 507024. Analytical method ( 3 ) — Identification: Pulsed-Field Gel Electrophoresis (PFGE) — Enumeration in the feed additive: Spread plate method on MRS agar (EN 15786) All animal species - - - 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 9 CFU/kg of easy and moderately difficult to ensile fresh material ( 4 ) . 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin, eyes and breathing protection. 16.3.2032 Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU of additive/kg of fresh material Category of technological additives. Functional group: silage additives 1k21017 Pediococcus pentosaceus IMI 507025 Additive composition: Preparation of Pediococcus pentosaceus IMI 507025 containing a minimum of 1 × 10 10 CFU/g additive. Solid form Characterisation of the active substance: Viable cells of Pediococcus pentosaceus IMI 507025. Analytical method ( 5 ) — Identification: Pulsed-Field Gel Electrophoresis (PFGE) — Enumeration in the feed additive: Spread plate method on MRS agar (EN 15786) All animal species - - - 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 9 CFU/kg of easy and moderately difficult to ensile fresh material ( 6 ) . 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin, eyes and breathing protection. 16.3.2032 Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU of additive/kg of fresh material Category of technological additives. Functional group: silage additives 1k21601 Lactiplantibacillus plantarum IMI 507026 Additive composition: Preparation of Lactiplantibacillus plantarum IMI 507026 containing a minimum of 1 × 10 10 CFU/g additive. Solid form Characterisation of the active substance: Viable cells of Lactiplantibacillus plantarum IMI 507026. Analytical method ( 7 ) — Identification: Pulsed-Field Gel Electrophoresis (PFGE) — Enumeration in the feed additives: Spread plate method on MRS agar (EN 15787) All animal species - - - 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 9 CFU/kg of easy and moderately difficult to ensile fresh material ( 8 ) . 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin, eyes and breathing protection. 16.3.2032 Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU of additive/kg of fresh material Category of technological additives. Functional group: silage additives 1k21602 Lactiplantibacillus plantarum IMI 507027 Additive composition: Preparation of Lactiplantibacillus plantarum IMI 507027 containing a minimum of 1 × 10 10 CFU/g additive. Solid form Characterisation of the active substance: Viable cells of Lactiplantibacillus plantarum IMI 507027. - Analytical method ( 9 ) — Identification: Pulsed-Field Gel Electrophoresis (PFGE) — Enumeration in the feed additives: Spread plate method on MRS agar (EN 15787) All animal species - - - 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 9 CFU/kg of easy and moderately difficult to ensile fresh material ( 10 ) . 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin, eyes and breathing protection. 16.3.2032 Identification number of the additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation CFU of additive/kg of fresh material Category of technological additives. Functional group: silage additives 1k21603 Lactiplantibacillus plantarum IMI 507028 Additive composition: Preparation of Lactiplantibacillus plantarum IMI 507028 containing a minimum of 1 × 10 10 CFU/g additive. Solid form Characterisation of the active substance: Viable cells of Lactiplantibacillus plantarum IMI 507028. Analytical method ( 11 ) — Identification: Pulsed-Field Gel Electrophoresis (PFGE) — Enumeration in the feed additives: Spread plate method on MRS agar (EN 15787) All animal species - - - 1. In the directions for use of the additive and premixtures, the storage conditions shall be indicated. 2. Minimum content of the additive when used without combination with other micro-organisms as silage additives: 1 × 10 9 CFU/kg of easy and moderately difficult to ensile fresh material ( 12 ) . 3. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including skin, eyes and breathing protection. 16.3.2032 ( 1 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports ( 2 ) Easy to ensile forage: > 3 % soluble carbohydrates in fresh material; moderately difficult to ensile forage: 1,5-3,0 % soluble carbohydrates in the fresh material in accordance with Commission Regulation (EC) No 429/2008 of 25 April 2008 on detailed rules for the implementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of applications and the assessment and the authorisation of feed additives ( OJ L 133, 22.5.2008, p. 1 ). ( 3 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports ( 4 ) Easy to ensile forage: > 3 % soluble carbohydrates in fresh material; moderately difficult to ensile forage: 1,5-3,0 % soluble carbohydrates in the fresh material in accordance with Commission Regulation (EC) No 429/2008 of 25 April 2008 on detailed rules for the implementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of applications and the assessment and the authorisation of feed additives ( OJ L 133, 22.5.2008, p. 1 ). ( 5 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports ( 6 ) Easy to ensile forage: > 3 % soluble carbohydrates in fresh material; moderately difficult to ensile forage: 1,5-3,0 % soluble carbohydrates in the fresh material in accordance with Commission Regulation (EC) No 429/2008 of 25 April 2008 on detailed rules for the implementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of applications and the assessment and the authorisation of feed additives ( OJ L 133, 22.5.2008, p. 1 ). ( 7 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports ( 8 ) Easy to ensile forage: > 3 % soluble carbohydrates in fresh material; moderately difficult to ensile forage: 1,5-3,0 % soluble carbohydrates in the fresh material in accordance with Commission Regulation (EC) No 429/2008 of 25 April 2008 on detailed rules for the implementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of applications and the assessment and the authorisation of feed additives ( OJ L 133, 22.5.2008, p. 1 ). ( 9 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports ( 10 ) Easy to ensile forage: > 3 % soluble carbohydrates in fresh material; moderately difficult to ensile forage: 1,5-3,0 % soluble carbohydrates in the fresh material in accordance with Commission Regulation (EC) No 429/2008 of 25 April 2008 on detailed rules for the implementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of applications and the assessment and the authorisation of feed additives ( OJ L 133, 22.5.2008, p. 1 ). ( 11 ) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports ( 12 ) Easy to ensile forage: > 3 % soluble carbohydrates in fresh material; moderately difficult to ensile forage: 1,5-3,0 % soluble carbohydrates in the fresh material in accordance with Commission Regulation (EC) No 429/2008 of 25 April 2008 on detailed rules for the implementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of applications and the assessment and the authorisation of feed additives ( OJ L 133, 22.5.2008, p. 1 ).
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