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Commission Implementing Regulation (EU) 2025/1254 of 25 June 2025 concerning the authorisation of riboflavin produced from Eremothecium ashbyi CCTCCM 2019833, in the form of a dried inactivated fermentation product, as a feed additive for all animal species

Commission Implementing Regulation (EU) 2025/1254 of 25 June 2025 concerning the authorisation of riboflavin produced from Eremothecium ashbyi CCTCCM 2019833, in the form of a dried inactivated fermentation product, as a feed additive for all animal species

Implementing Regulation (EU) 2025/1254 · Regulation · 3 articles

Data as of 2026-07-04 · Compiled from an official source version. Later amendments or repeals may not be reflected; the official text prevails. · Read the official text ↗

Authorisation

Article 1

The substance specified in the Annex, belonging to the additive category ‘nutritional additives’ and to the functional group ‘vitamins, pro-vitamins and chemically well-defined substances having similar effect’, is authorised as an additive in animal nutrition, subject to the conditions laid down in that Annex.

Entry into force

Article 2

This Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.

Supplementary provisions

ANNEXSupplementary provisions

ANNEX Identification number of the feed additive Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation mg of the additive/kg of complete feedingstuff with a moisture content of 12 % Category: nutritional additives. Functional group: vitamins, pro-vitamins and chemically well-defined substances having similar effect 3a827 ‘Riboflavin’ or ‘Vitamin B 2 ’ Additive composition Riboflavin produced from Eremothecium ashbyi CCTCCM 2019833, in the form of a dried inactivated fermentation product, containing a minimum of 5 % riboflavin. Moisture ≤ 7 % Solid form Characterisation of active substance Riboflavin produced from Eremothecium ashbyi CCTCCM 2019833 Chemical formula: C 17 H 20 N 4 O 6 CAS number: 83–88–5 Analytical method  ( 1 ) For the determination of riboflavin in the feed additive: — High Performance Liquid Chromatography with Fluorescence detection (HPLC-FLD); or — High Performance Liquid Chromatography with UV detection (HPLC-UV) — VDLUFA Bd. III, 13.9.1. For the determination of riboflavin in premixtures: — High Performance Liquid Chromatography with UV detection (HPLC-UV) — VDLUFA Bd. III, 13.9.1. For the determination of riboflavin (as total vitamin B2) in compound feed: — High Performance Liquid Chromatography with Fluorescence detection (HPLC-FLD) — EN 14152. All animal species — —   1. The directions for use of the additive and premixture shall indicate the storage conditions, the stability to heat treatment. 2. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks. Where those risks cannot be eliminated by such procedures and measures, the additive and premixtures shall be used with appropriate personal protective equipment, including breathing protection. 16 July 2035 ( 1 )   Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en .

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Source: EUR-Lex (Publications Office of the EU), © European Union, reuse permitted under Commission Decision 2011/833/EU.

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