ANNEX IISupplementary provisions
ANNEX II No or EC No Additive Chemical formula, description Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation Units of activity/kg of complete feedingstuff Enzymes 54 Endo-1,3(4)-beta-glucanase EC 3.2.1.6 Endo-1,4-beta-glucanase EC 3.2.1.4 Alpha-amylase EC 3.2.1.1 Endo-1,4-beta-xylanase EC 3.2.1.8 Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and endo-1,4-beta-xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of: Endo-1,3(4)-beta-glucanase: 10 000 U ( 1 ) /g Endo-1,4-beta-glucanase: 120 000 U ( 2 ) /g Alpha-amylase: 400 U ( 3 ) /g Endo-1,4-beta-xylanase: 210 000 U ( 4 ) /g Laying hens — Endo-1,3(4)-beta-glucanase: 500 U — 1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting 2. Recommended dose per kg of complete feedingstuff: endo-1,3(4)-beta-glucanase: 500-1 500 U endo-1,4-beta-glucanase: 6 000 -18 000 U alpha-amylase: 20-60 U endo-1,4-beta-xylanase: 10 500 -31 500 U 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30 % wheat 29.9.2009 endo-1,4-beta-glucanase: 6 000 U — alpha-amylase: 20 U — endo-1,4-beta-xylanase: 10 500 U — 60 Endo-1,4-beta-xylanase EC 3.2.1.8 Endo-1,3(4)-beta-glucanase EC 3.2.1.6 Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activity of: Liquid form: Endo-1,4-beta-xylanase: 5 000 U ( 5 ) /ml Endo-1,3(4)-beta-glucanase: 50 U ( 6 ) /ml Turkeys for fattening — Endo-1,4-beta-xylanase: 1 250 U — 1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelletting 2. Recommended dose per kg of complete feedingstuff: endo-1,4-beta-xylanase: 1 250 -2 500 U endo-1,3(4)-beta-glucanase: 12-25 U 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 20 % barley and 40 % wheat 29.9.2009 endo-1,3(4)-beta-glucanase: 12 U — 62 Endo-1,4-beta-xylanase EC 3.2.1.8 Preparation of endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 109.713) having a minimum activity of: Solid form: 5 600 TXU ( 7 ) /g Liquid form 5 600 TXU/ml Chickens for fattening — 200 TXU — 1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelletting 2. Recommended dose per kg of complete feedingstuff: 400-800 TXU 3. For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 40 % wheat 29.9.2009 ( 1 ) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30 °C. ( 2 ) 1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 4,8 and 50 °C. ( 3 ) 1 U is the amount of enzyme which hydrolyses 1 micromole of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 7,5 and 37 °C. ( 4 ) 1 U is the amount of enzyme which liberates 0,0067 micromoles of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 5,3 and 50 °C. ( 5 ) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C. ( 6 ) 1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C. ( 7 ) 1 TXU is the amount of enzyme which liberates 5 micromoles of reducing sugars (xylose equivalents) from wheat arabinoxylan per minute at pH 3,5 and 55 °C.