Commission Regulation (EC) No 1458/2005 of 8 September 2005 concerning the permanent and provisional authorisations of certain additives in feedingstuffs and the provisional authorisation of new uses of certain additives already authorised in feedingstuffs (Text with EEA relevance)

• CELEX: Regulation (EC) No 1458/2005
• Articles: 5

Article 1

The preparation belonging to the group ‘Enzymes’, as specified in Annex I, is authorised for use without a time limit as additive in animal nutrition under the conditions laid down in that Annex.

Article 2

The preparations belonging to the group ‘Enzymes’, as specified in Annex II, are authorised for use for four years as additive in animal nutrition under the conditions laid down in that Annex.

Article 3

This Regulation shall enter into force on the 20th day following its publication in the Official Journal of the European Union .

Schedules & Appendices

ANNEX I

ANNEX I

EC No

Additive

Chemical formula, description

Species or category of animal

Maximum age

Minimum content

Maximum content

Other provisions

End of period of authorisation

Units of activity/kg of complete feedingstuff

Enzymes

E 1634

Endo-1,3(4)-beta-glucanase EC 3.2.1.6

Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus niger (MUCL 39199) having a minimum activity of:

Solid form:

1 500  AGL  ( 1 ) /g

Liquid form:

200 AGL/ml

Chickens for fattening

—

25  AGL

—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2.

Recommended dose per kg of complete feedingstuff:

25-100 AGL

3.

For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 50 % barley

Without a time limit

( 1 )   1 AGL is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 4,6 and 30 °C.

ANNEX II

ANNEX II

No or EC No

Additive

Chemical formula, description

Species or category of animal

Maximum age

Minimum content

Maximum content

Other provisions

End of period of authorisation

Units of activity/kg of complete feedingstuff

Enzymes

54

Endo-1,3(4)-beta-glucanase EC 3.2.1.6

Endo-1,4-beta-glucanase EC 3.2.1.4

Alpha-amylase EC 3.2.1.1

Endo-1,4-beta-xylanase EC 3.2.1.8

Preparation of endo-1,3(4)-beta-glucanase produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94), alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553) and endo-1,4-beta-xylanase produced by Trichoderma viride (NIBH FERM BP 4842) having a minimum activity of:

Endo-1,3(4)-beta-glucanase: 10 000  U  ( 1 ) /g

Endo-1,4-beta-glucanase: 120 000  U  ( 2 ) /g

Alpha-amylase: 400 U  ( 3 ) /g

Endo-1,4-beta-xylanase: 210 000  U  ( 4 ) /g

Laying hens

—

Endo-1,3(4)-beta-glucanase: 500 U

—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting

2.

Recommended dose per kg of complete feedingstuff:

endo-1,3(4)-beta-glucanase: 500-1 500  U

endo-1,4-beta-glucanase: 6 000 -18 000  U

alpha-amylase: 20-60 U

endo-1,4-beta-xylanase: 10 500 -31 500  U

3.

For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 30 % wheat

29.9.2009

endo-1,4-beta-glucanase: 6 000  U

—

alpha-amylase: 20 U

—

endo-1,4-beta-xylanase: 10 500  U

—

60

Endo-1,4-beta-xylanase EC 3.2.1.8

Endo-1,3(4)-beta-glucanase EC 3.2.1.6

Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activity of:

Liquid form:

Endo-1,4-beta-xylanase: 5 000  U  ( 5 ) /ml

Endo-1,3(4)-beta-glucanase: 50 U  ( 6 ) /ml

Turkeys for fattening

—

Endo-1,4-beta-xylanase: 1 250  U

—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelletting

2.

Recommended dose per kg of complete feedingstuff:

endo-1,4-beta-xylanase: 1 250 -2 500  U

endo-1,3(4)-beta-glucanase: 12-25 U

3.

For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 20 % barley and 40 % wheat

29.9.2009

endo-1,3(4)-beta-glucanase: 12 U

—

62

Endo-1,4-beta-xylanase EC 3.2.1.8

Preparation of endo-1,4-beta-xylanase produced by Aspergillus niger (CBS 109.713) having a minimum activity of:

Solid form:

5 600  TXU  ( 7 ) /g

Liquid form

5 600 TXU/ml

Chickens for fattening

—

200  TXU

—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelletting

2.

Recommended dose per kg of complete feedingstuff: 400-800 TXU

3.

For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 40 % wheat

29.9.2009

( 1 )   1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 7,5 and 30 °C.

( 2 )   1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 4,8 and 50 °C.

( 3 )   1 U is the amount of enzyme which hydrolyses 1 micromole of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 7,5 and 37 °C.

( 4 )   1 U is the amount of enzyme which liberates 0,0067 micromoles of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 5,3 and 50 °C.

( 5 )   1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.

( 6 )   1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.

( 7 )   1 TXU is the amount of enzyme which liberates 5 micromoles of reducing sugars (xylose equivalents) from wheat arabinoxylan per minute at pH 3,5 and 55 °C.