ANNEX ISupplementary provisions
ANNEX I SPECIFICATIONS OF YEAST ( SACCHAROMYCES CEREVISIAE ) BETA-GLUCANS Description Beta-glucans are complex, high molecular mass (100–200 kDa) polysaccharides, found in the cell wall of many yeasts and cereals. The chemical name for ‘yeast beta-glucans’ is (1-3), (1-6)-β-D-glucans. Beta-glucans consist of a backbone of β-1-3-linked glucose residues that are branched by β-1-6-linkages, to which chitin and mannoproteins are linked by β-1-4-bonds. This novel food is a highly purified (1,3)-(1,6)-β-D-glucan isolated from yeast Saccharomyces cerevisiae , insoluble in water, but dispersible in many liquid matrices. Specifications of yeast ( Saccharomyces cerevisiae ) beta-glucans Parameter Specifications values Solubility Insoluble in water but dispersible in many liquid matrices Chemical data (1,3)-(1,6)-β-D-Glucan > 80 % Ash < 2 % Moisture < 6 % Protein < 4 % Total fat < 3 % Microbiological data Total plate count < 1 000 CFU/g Enterobacteriaceae < 100 CFU/g Total coliforms < 10 CFU/g Yeast < 25 CFU/g Mould < 25 CFU/g Salmonella ssp. Absent in 25 g Escherichia coli Absent in 1 g Bacillus cereus < 100 CFU/g Staphylococcus aureus Absent in 1 g Heavy metals Lead < 0,2 mg/g Arsenic < 0,2 mg/g Mercury < 0,1 mg/g Cadmium < 0,1 mg/g