ANNEX II
DETERMINATION OF THE TOTAL WATER CONTENT OF POULTRYMEAT CUTS REFERRED TO IN ARTICLE 8(1)
(Chemical test)
1. OBJECT AND SCOPE
The method to determine the total water content of certain poultry cuts involves determination of the water and protein contents of samples from the homogenised poultry cuts. The total water content as determined is compared with the limit value given by the formulae indicated in Annex VIII to Delegated Regulation (EU) 2026/343, to determine whether or not excess water has been taken up during processing. If the analyst suspects the presence of any substance which may interfere with the assessment, it is for him or her to take the necessary appropriate precautions.
2. DEFINITIONS AND SAMPLING PROCEDURES
The definitions provided for in Article 2, point (2), of Delegated Regulation (EU) 2026/343 are applicable to the poultry cuts referred to in Article 14(2) of that Delegated Regulation. Sample sizes for different types of poultry meat correspond to the following shares:
(a)
chicken breast: half of the breast;
(b)
chicken breast fillet: half of the boned breast without skin;
(c)
turkey breast, turkey breast fillet and boned leg meat: portions of about 100 g;
(d)
other cuts: whole cut as defined in Article 2, point (2), of Delegated Regulation (EU) 2026/343.
In the case of frozen or quick-frozen bulk products (cuts not individually packed) the large packs from which samples are to be taken shall be kept at 0 °C until individual cuts can be removed.
3. PRINCIPLE
Water and protein contents are determined in accordance with recognised ISO (International Organisation for Standardisation) methods or other methods of analysis approved by the Council of the European Union.
The highest permissible total water content of the poultry cuts is determined from the protein content of the cuts, which can be related to the physiological water content.
4. APPARATUS AND REAGENTS
4.1.
Scales for weighing the cuts and wrappings, accurate to at least 1 g.
4.2.
Meat-axe or saw for cutting cuts into pieces of appropriate size for the mincer.
4.3.
Heavy-duty mincing machine and blender capable of homogenising poultry cuts or parts thereof.
NB:
No special mincer is recommended. It should have sufficient power to mince frozen or quick-frozen meat and bones to produce a homogeneous mixture corresponding to that obtained from a mincer fitted with a 4-mm hole disc.
4.4.
Apparatus as specified in ISO 1442, for the determination of water content.
4.5.
Apparatus as specified in ISO 937, for the determination of protein content.
5. METHOD
5.1.
Five cuts are taken at random from the quantity of poultry cuts to be checked and kept frozen or refrigerated as the case may be until analysis in accordance with points 5.2 to 5.6 begins.
Samples from frozen or quick-frozen bulk products referred to under point 2 shall be kept at 0 °C until analysis begins.
The analysis shall concern either each of the five cuts separately or a composite sample of the five cuts.
5.2.
The preparation is commenced within one hour following the removal of the cuts from the freezer or refrigerator.
5.3.
(a)
The outside of the pack is wiped to remove superficial ice and water. Each cut is weighed and removed from any wrapping material. After cutting up the cuts into smaller pieces, the weight of the poultry cut is determined to the nearest gram after deduction of the weight of any wrapping material removed, to give ‘P 1 ’.
(b)
In the case of a composite sample analysis, the total weight of the five cuts, prepared in accordance with point 5.3(a), is determined to give ‘P 5 ’.
5.4.
(a)
The whole cut of which the weight is P 1 , is minced in a mincer as specified under point 4.3 (and, if necessary, mixed with the use of a blender as well) to obtain a homogeneous material from which a sample representative of each cut shall then be taken.
(b)
In the case of a composite sample analysis, all five cuts of which the weight is P 5 are minced in a mincer as specified under point 4.3 (and, if necessary, mixed with the use of a blender as well) to obtain a homogeneous material from which two samples representative of the five cuts shall then be taken.
The two samples are analysed as described in points 5.5 and 5.6.
5.5.
A sample of the homogenised material is taken and used immediately to determine the water content in accordance with ISO 1442 to give the water content ‘a %’.
5.6.
A sample of the homogenised material is also taken and used immediately to determine the nitrogen content in accordance with ISO 937. This nitrogen content is converted to crude protein content ‘b %’ by multiplying it by the factor 6,25.
6. CALCULATION OF RESULTS
6.1.
(a)
The weight of water (W) in each cut is given by aP 1 /100 and the weight of protein (RP) by bP 1 /100, both of which are expressed in grams.
The sums of the weights of water (W 5 ) and the weights of protein (RP 5 ) in the five cuts analysed are determined.
(b)
In the case of a composite sample analysis, the average content of water and protein from the two samples analysed is determined to give a % and b %, respectively. The weight of the water (W 5 ) in the five cuts is given by aP 5 /100, and the weight of protein (RP 5 ) by bP 5 /100, both of which are expressed in grams.
6.2.
The average weight of water (W A ) and protein (RP A ) is calculated by dividing W 5 and RP 5 respectively, by five.
6.3.
The mean physiological W/RP ratio as determined by this method is as follows:
chicken breast fillet: 3,19 ± 0,12,
chicken legs and leg quarters: 3,78 ± 0,19,
turkey breast fillet: 3,05 ± 0,15,
turkey legs: 3,58 ± 0,15,
deboned turkey leg meat: 3,65 ± 0,17.